Listeria PatternAlert™ Assay

Rheonix, Inc. announces the launch of its Listeria PatternAlert™ assay, a breakthrough method for rapidly identifying molecular patterns from Listeria strains. The method will assist food producers in identifying harborage sites for persistent Listeria and in tracing back sources of contamination. The Listeria PatternAlert assay provides results in just six hours after a presumptive positive screen, enabling users to take rapid action to reduce the risk of contamination and recall.

Current strain typing methods take up to two weeks to complete and require an isolate in pure culture. The Listeria PatternAlert assay is performed using the fully automated Encompass Optimum™ workstation, enabling users to detect molecular patterns in just six hours directly from a positive enriched sample, without the need for an isolate.

How it works

The Listeria PatternAlert assay detects the presence or absence of independently occurring genetic targets that can sort Listeria into thousands of potential patterns. Each pattern generated by the assay encompasses a group of strains and may include multiple species of Listeria. The discriminatory power of the PatternAlert assay has been carefully calibrated to enable users to make informed decisions based on molecular patterns, without providing a strain level characterization equivalent to whole genome sequencing or pulsed field gel electrophoresis.

With the Listeria PatternAlert assay, once a presumptive positive result has been obtained using an existing screening method, a sample from the initial enrichment can be processed directly on the Encompass Optimum workstation, without the need for an isolate. This method provides users with a rapid, cost-effective tool to aid in identifying, tracking and addressing recurring Listeria.

Advantages

  • Rapid identification of recurring Listeria patterns
  • Actionable results in 6 hours
  • Direct from positive enrichment
  • No isolate required
  • Runs on fully automated Encompass Optimum™ workstation
  • User-specific database of patterns

Why use the Listeria PatternAlert assay?

Persistent strains that find a harborage site in a food facility can significantly increase the risk of broader contamination, and are a target of increasing regulatory scrutiny. Food producers routinely screen for the presence of Listeria and take corrective action when positive samples are found. Until now, however, obtaining additional information on Listeria strains or populations in a sample was time-consuming and required an isolate in pure culture. The Listeria PatternAlert assay provides results in just six hours after a presumptive positive screen, without the need for an isolate, enabling users to take rapid action to reduce the risk of contamination and recall.

Launching at IAFP 2018!

Rheonix will showcase the Listeria PatternAlert assay for use on the Encompass Optimum workstation at IAFP at booth 926. Morgan Wallace, Ph.D., scientific director for applied markets at Rheonix, will also give a technical presentation titled “Development of a Molecular Listeria Pattern Recognition Assay, a Novel Rapid Method for Identifying Resident Listeria Wednesday, July 11, at 2:00 p.m. in Room 151 D-G, Salt Palace Convention Center.

Getting more information from your sample has never been easier!

 

Product Information

Rheonix Listeria PatternAlert™ Assay

Contact Us

To learn more about Rheonix and the Listeria PatternAlert™ Assay, contact us via email at foodsafety@rheonix.com.

Rheonix, Inc.
10 Brown Road, Suite 103
Ithaca, NY 14850 USA

(607) 257-1242 ext. 160