Enhanced Beer SpoilerAlert™ Assay Detection

Beer SpoilerAlert™ assay has a built-in 3x9 detection array for a total of 27 probe locations. There are 6 probe spots set aside for process and amplification controls for each sample. The remaining 21 target capture probes behave as follows:

• Unique to a specific primer target
  • Saccharomyces cerevisiae variant diastaticus
  • Hop resistance genes (horA, horC, bsrA, bsrB)
• Combine in a fingerprint to allow for speciation of a genus
  • Brettanomyces: bruxellensis, B. acidodurans, B. anomalus, B. custersianus, and B. naardenensis
• Identify all members of a genus without distinction
  • Pediococcus: claussenii, P. damnosus, P. inopinatus, P. acidilactici, P. cellicola, P. parvulus, P. pentosaceus, and P. stilesii
  • Megasphaera: cerevisiae, M. elsdenii, M. paucivorans, M. sueciensis
  • Pectinatus: cerevisiiphilus, P. frisingensis, P. haikarae
• Identify individual subsets of a genus
  • Lactobacillus:  acetotolerans, L. acidophilus, L. agilis, L. amylolyticus, L. amylovorus, L. backii, L. brevis, L. buchneri, L. casei, L. collinoides, L. coryniformis, L. crispatus, L. curieae, L. curvatus, L. delbrueckii, L. fermentum, L. gallinarum, L. gasseri, L. harbinensis, L. helveticus, L. jensenii, L. johnsonii, L. lindneri, L. malfermentans, L. parabuchneri, L. paracasei, L. paracollinoides, L. paraplantarum, L. paucivorans, L. pentosus, L. perolens, L. plantarum, L. rennini, L. reuteri, L. rhamnosus, L. rossiae, L. ruminis, L. sakei, and  L. salivarius

Below is an example of the run report generated at the end of a run, followed by the key explaining the results.